Analytical Chemistry Lesson of the Day – Method Validation in Quality Assurance
June 22, 2015 1 Comment
When developing any method in analytical chemistry, it must meet several criteria to ensure that it accomplishes its intended objective at or above an acceptable standard. This process is called method validation, and it has the following criteria* in the pharmaceutical industry:
- limit of detection
- limit of quantitation
As I will note in future Chemistry Lessons of the Day, these words are used differently between statistics and chemistry.
*These criteria are taken from Page 723 of the 6th edition of “Quantitative Chemical Analysis” by Daniel C. Harris (2003).
**The Food and Drug Administration does not list robustness as a typical characteristic of method validation. (See Section B on Page 7 of its “Guidance for Industry Analytical Procedures and Methods Validation for Drugs and Biologics“.) However, it does mention robustness several times as an important characteristic that “should be evaluated” during the “early stages of method development”.